IUI (Intra Uterine Insemination)

Best IUI Treatment in Pune

IUI (Intra Uterine Insemination)

This procedure is performed in cases where the husband’s sperm count or motility is affected. Generally single IUI is performed with in a day’s time of release of egg from the ovary (ovulation )of the female partner. Some times the procedure of insemination is done twice, just before and after the ovulation.

IUI has two steps , 1) collection and processing of semen and 2) insemination of the processed sperm sample into female partner.

1) Collection and processing of the semen sample.

Preferably semen sample is collected in the IVF center after 2-3 days of abstinence as per comfort of the male partner. Sample is to be obtained following hand masturbation and collected in a container provided by the center.

The method and thus the time required for sperm processing depends on the native sperm count. Generally it takes 45 minutes to one hour.

2) Insemination into female partner

The semen sample that is processed in pushed into uterus of the female partner. There is no anesthesia required for the process. the lady can continue all the routine activities before and after the process.

iui intrauterine insemination

We do around 40 IUI cycles every month and our IUI success rate is 60 %.

Blastocyst transfer

Embryos are cultured for five to six days in the laboratory incubator to the blastocyst stage, before they are transferred to the womb. At this stage, one or two of the best quality blastocysts are selected and then implanted into the woman’s womb

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Assisted hatching

The embryo is covered by a layer called zona pellucid. The layer is very thick in some patients, making it difficult for the embryo to come out of zona and implant in the uterus. This is the reason why in some patients the zona is drilled with laser.

Assisted hatching may also be done in patients in whom the zona has become hard post vitrification- thaw. Assisted hatching has been found to help with IVF success in poor prognosis patients, whose embryos lack sufficient energy to complete the hatching process. The technique may be suggested for women older than 38 years of age, have undergone two or more failed IVF cycles or have poor embryo quality.

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Pre-implantation genetic testing (PGT) is a technique used prior to implantation to help identify genetic defects within embryos created through IVF to prevent certain diseases or disorders from being passed on to the child. PGT begins with the normal process of IVF that includes egg retrieval and fertilization in a laboratory. Over the next three days the embryo divides into eight cells. One or two of these cells are removed from the embryo and evaluated in an advanced genetic testing laboratory to determine if the inheritance of a problematic gene is present in the embryo. Once the PGT procedure has been performed and embryos free of genetic problems have been identified, the embryo is placed back in the uterus, and implantation will be attempted. Pre-implantation genetic diagnosis (PGD) can test for 100+ genetic conditions that include genetic diseases such as thalassemia and hemophilia. As the procedure is performed before implantation, there is maximum chance of disease free baby being born. PGD is recommended for: - Carriers of sex-linked genetic disorders - Carriers of single gene disorders - Those with known chromosomal disorder Pre-implantation genetic screening (PGS) is offered to: - Patients where there is no known genetic problem, yet cause of infertility is not known. - Women age 35 and over. - Women experiencing recurring pregnancy loss. - Women with multiple failed fertility treatments. - Rule out aneuploid embryos. While pre-implantation genetic testing helps reduce the chances of conceiving a child with a genetic disorder, it cannot completely eliminate this risk.


  • 1. Parental Gene Analysis: Parents may be carriers of disease genes or the embryo may develop a de-novo mutation
  • 2. ICSI: Individual sperm is injected in individual oocyte to create an embryo.
  • 3. Embryo Biopsy: On day 5, five to eight ells of the trophectoderm are removed and sent for analysis. The embryos are vitrified individually.
  • 4. DNA Isolation: DNA from the cells is isolated.
  • 5. Molecular Genetic Testing: Using next generation sequencing, the isolated DNA is analysed.
  • 6. Embryo Identification & Transfer: Affected (embryos with mutation) & non-affected embryos are identified. non affected embryos are transferred back into the woman.

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